syber green primers  (Thermo Fisher)

Journal: Scientific Reports

Article Title: Innate And Adaptive Immunity are Progressively Activated in Parallel with Renal Injury in the 5/6 Renal Ablation Model

doi: 10.1038/s41598-017-02915-6

Figure Lengend Snippet: Heat map representation of PCR-array analysis ( A ) with the respective fold change value (mean) of each studied gene ( B ). Venn diagrams ( C ) illustrate the relative participation of overexpressed genes related to innate and adaptive immune responses at each time point. For these experiments, cDNA samples were obtained from nine different animals per group: Sham, Nx 15d, Nx 60d and Nx 120d. These cDNA was than distributed among three pools per group, containing genetic material of three different animals each. For statistical analysis we considered N = 3. Differences between Nx and Sham were assessed by Student’s t-test (*p < 0.05 vs. Sham), using the Rt2 Profiler PCR Array Analysis software, Version 3.5™ (Qiagen).

Article Snippet: The cDNAs were mixed with the appropriate PCR master mix buffer (Rt2 Syber Green ROX qPCR Primer Assay QiagenN® #330523) and analyzed on specific array plates (Rat Innate & Adaptive Immune Responses Rt2 ProfilerTM PCR, Qiagen®, #330231-PARN052Zc).

Techniques: Software

Journal: Scientific Reports

Article Title: Innate And Adaptive Immunity are Progressively Activated in Parallel with Renal Injury in the 5/6 Renal Ablation Model

doi: 10.1038/s41598-017-02915-6

Figure Lengend Snippet: Bar graph representation of the PCR-array analysis of genes related to the TLR and inflammasome pathways throughout the study. For these experiments, cDNA samples were obtained from nine different animals per group: Sham, Nx 15d, Nx 60d and Nx 120d. These cDNA was than distributed among three pools per group, containing genetic material of three different animals each. For statistical analysis we considered N = 3. Results are presented as mean of fold change, compared to Sham animals. Differences between Nx and Sham were assessed by Student’s t-test (*p < 0.05 vs. Sham), using the Rt2 Profiler PCR Array Analysis software, Version 3.5™ (Qiagen).

Article Snippet: The cDNAs were mixed with the appropriate PCR master mix buffer (Rt2 Syber Green ROX qPCR Primer Assay QiagenN® #330523) and analyzed on specific array plates (Rat Innate & Adaptive Immune Responses Rt2 ProfilerTM PCR, Qiagen®, #330231-PARN052Zc).

Techniques: Software

Journal: Scientific Reports

Article Title: Innate And Adaptive Immunity are Progressively Activated in Parallel with Renal Injury in the 5/6 Renal Ablation Model

doi: 10.1038/s41598-017-02915-6

Figure Lengend Snippet: Quantitative PCR analysis (qPCR) for Tlr4 ( A ), Nlrp3 ( B ), Casp1 ( C ) and Il1b ( D ) gene expression. Results are presented as median plus range. The number of analyzed animals is represented next to the name of each group. Differences among groups were analyzed by one-way ANOVA with Newman-Keuls post test. a p < 0.05 vs. Sham, b p < 0.05 vs. Nx 15d, c p < 0.05 vs. Nx 60d.

Article Snippet: The cDNAs were mixed with the appropriate PCR master mix buffer (Rt2 Syber Green ROX qPCR Primer Assay QiagenN® #330523) and analyzed on specific array plates (Rat Innate & Adaptive Immune Responses Rt2 ProfilerTM PCR, Qiagen®, #330231-PARN052Zc).

Techniques: Real-time Polymerase Chain Reaction, Gene Expression

Journal: Scientific Reports

Article Title: Innate And Adaptive Immunity are Progressively Activated in Parallel with Renal Injury in the 5/6 Renal Ablation Model

doi: 10.1038/s41598-017-02915-6

Figure Lengend Snippet: Bar graphs representing the expression of genes related to ( A ) Macrophage (Mϕ) M1 ( Cd80, Tlr2, Tlr4, Il6 ) and M2 ( Ccl3, Tlr1, Tlr5, Il10 ) subsets and to ( B ) T-Lymphocyte (T-Ly) Th1 ( Ccr5, Cxcr3, Tbx21 ), Th2 ( Gata3, Il13, Il5 ), Th17 ( Sat3 , Ccr6 ) and Treg ( Ccr4 , Foxp3 ) subsets in the renal parenchyma of Nx rats, using PCR-array analysis. For these experiments, cDNA samples were obtained from nine different animals per group: Sham, Nx 15d, Nx 60d and Nx 120d. These cDNA was than distributed among three pools per group, containing genetic material of three different animals each. For statistical analysis we considered N = 3. Results are presented as mean of fold change, and differences between Nx and Sham were assessed by Student’s t-test (*p < 0.05 vs. Sham), and analyzed with the Rt2 Profiler PCR Array Analysis software, Version 3.5™ (Qiagen).

Article Snippet: The cDNAs were mixed with the appropriate PCR master mix buffer (Rt2 Syber Green ROX qPCR Primer Assay QiagenN® #330523) and analyzed on specific array plates (Rat Innate & Adaptive Immune Responses Rt2 ProfilerTM PCR, Qiagen®, #330231-PARN052Zc).

Techniques: Expressing, Software